Esmail Khazaei; Mikhalil Piruzifard; Fariba Zeynali; Mohammad Alizadeh khaled abad
Abstract
Introduction: Meat and meat products are important sources of protein, fat, essential amino acids, minerals and vitamins, and other nutrients. Bologna sausage, a cooked meat product, is one of the most accepted processed meat products and is consumed and enjoyed worldwide. Among the meat products, sausage ...
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Introduction: Meat and meat products are important sources of protein, fat, essential amino acids, minerals and vitamins, and other nutrients. Bologna sausage, a cooked meat product, is one of the most accepted processed meat products and is consumed and enjoyed worldwide. Among the meat products, sausage and bologna in vacuum packages due to their variety, easy to use applications and being economical, have become commonplace. The most important problem with the production, storage, and sale of this kind of meat products is their syneresis in the vacuum packages. In addition to an unpleasant appearance, syneresis leads to accelerating microbial growth and undesirable changes in the flavor and odor of the product.The addition of functional ingredients helps to modify the overall technological and sensorial characteristics of a meat system such as water holding capacity (WHC), fat holding capacity, and texture properties and decrease the syneresis. Syneresis and purge are seen as a result of retrogradation of starch and this is very common in sliced and vacuum-packed meat products. Storage of meat products containing high-amylose starches, at low temperatures from around –1 °C to 0 °C for a prolonged time also favors retrogradation. The level of retrogradation depends on the type of starch and wheat starch demonstrating the greatest tendency towards retrogradation. Chitosan is a natural cationic polysaccharide antibacterial activity and antioxidant properties in muscle foods during storage. The aim of this study was Investigation the addition of functional ingredients such as chitosan and potato modified starch in the formulation on the amount of syneresis, pH, total microbial count and sensory evaluation in two type of products including beef ham and chicken ham were examined during storage. Then, the effect of vacuum packaging type and the amount of vacuum created in the package on the amount of syneresis were investigated. Material and methods: In this study, first various factors such as three levels of chitosan (0, 0.3 and 0.6%) and replace the wheat starch with three levels of potato modified (0, 5 and 10%) in the formulation on the amount of syneresis, pH, total microbial count and sensory evaluation in two type of products including beef ham and chicken ham were examined during storage time (1, 15 and 30 days). In this part of the study, samples with the least syneresis were selected. After that various factors of packaging such as two types of vacuum packaging(simple vacuum pack and skin pack) and the amount of vacuum(5 and 30 millibars) created in the package on the amount of syneresis were examined during storage(1, 15 and 30 days). Wheat starch, potato starch, and chitosan were purchased, respectively, from Faradane Company, KMC Company, and Sinaseven Company. The chicken meat was purchased from Fileh Amol Company. The beef was purchased from the Minerva Company of Brazil. All steps of samples’ preparation were performed in the Kalleh Amol Meat company. The chicken ham samples in this study consisted of 90% of the chicken meat and beef ham samples consisted of 90% of beef. Vacuum packaging was done using the machines which were manufactured by the German company Multivac. Syneresis was measured using the method suggested by Cesare et al. (e 2013). The initial weight of the ham slices before vacuum-packed was measured (P1); on the day of the analysis, the packaging was opened and the slices were dried with a disposable absorbent towel, then the dried ham slices were weighed again (P2). Syneresis was expressed as Syneresis= (P1- P2) ×100/P1. This study was conducted in a completely randomized design and Duncan multi-ranged test was used to determine differences between samples at 95% confidence level. Results and discussion: According to the results of analysis of variance, there was no significant difference between the syneresis of beef and chicken ham. The effect of storage time was significant on the syneresis and the syneresis rate increased with increasing storage time. Also, the results showed that adding chitosan and potato modified starch significantly (p<0.05) reduced the product's syneresis during storage. Potato starch has more water storage capacity and more amylopectin than wheat starch. For this reason, potato starch has a weaker retrogradation and less syneresis. Chitosan is a cationic polysaccharide with water-absorbing polar groups. Chitosan also was prevented from decreasing the product’s pH during storage time, that this result showed the antimicrobial effect of chitosan on acid lactic bacteria, especially in 15 and 30 days of storage. For that reason, chitosan prevents product’s pH to reach to its isoelectric pH. According to the sensory evaluation results, during the maintenance period, the sensory quality decreased significantly while chitosan had a significant positive effect on sensory parameters during storage time (P<0.05). The type of vacuum packaging and the amount of vacuum created in the package showed a significant effect on the amount of samples’ syneresis. Skin packaging showed less syneresis rates than simple vacuum packages. This result can be due to two reasons: one is the shrinkage of the product in the package, and the other is the amount of the film packing tangency on the surface of the products. So that by increasing the amount of vacuum, syneresis value increased significantly. Increasing the negative pressure inside the package caused increase the mechanical pressure by the packaging film on the product. In total, it can be concluded that with the addition of modified potato starch and chitosan to the formulation, as well as the use of lower-vacuum Skin packaging, the amount of syneresis of meat products can be significantly reduced.
Esmail Khazaei; Fakhri Shahidi; Seyed Ali Mortazavi; Mohebbat Mohebbi
Abstract
Introduction: Kiwifruit (Actinidia sp.) has many appealing properties for consumers such as flavor, color, and nutritional content, especially vitamin C. Kiwi seems to be used as one of the major ingredients within the formulation of this category of foods.Microalgae are nutritional and innovative natural ...
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Introduction: Kiwifruit (Actinidia sp.) has many appealing properties for consumers such as flavor, color, and nutritional content, especially vitamin C. Kiwi seems to be used as one of the major ingredients within the formulation of this category of foods.Microalgae are nutritional and innovative natural sources that can be used in the development of novel foods. Among the known species of algae, Chlorella vulgaris and Spirulinaplatensis are common edible microalgae that have no side effects on health of human. The amino acid, carbohydrate, and fatty acid profiles of these microalgae are very similar to those of other food materials. Spirulina is a multicellular filamentous blue-green microalga, which was introduced as generally recognized as safe (GRAS) after being approved by food and drug administration (FDA). Spirulina can also be incorporated into common food preparations such as pastas, biscuit, bread, snack,pastille, candy, yoghurt, soft drink, causing health-promotion that are associated with microalgae biomass.The aim of this study was to investigate the possibility of producing new product based on kiwifruit and its enrichment with Spirulinaplatensis microalgae so that this product could replace common snacks, especially the pastilles containing synthetic colors and flavors, which have increasingly been used day by day despite having a low nutritional value and causing a variety of side effects. The role of pHysicochemical and sensory properties is very important in producing new products which can help producers design the products suitably. Therefore, some of the pHysicochemical properties of the kiwi base pastille were examined in this study, as well as the formulation and enrichment of it with Spirulinaplatensis. Material and methods: The ingredients of the formulations consist of 65% w/w kiwifruit puree, 30% w/w sweeteners (sugar, powdered glucose, invert syrup and sorbitol), 0.25% w/w high methoxyl pectin, 0.5% Agar, 0.5% Guar and Spirulinaplatensis(0, 0.25, 0.5, 1 and 2% w/w) as well.In order to produce fruit pastille based on kiwifruit puree, the kiwifruits were fist washed up, peeled and cut into pieces. Then the pieces were grinded. The prepared puree was mixed with Spirulinaplatensis, hydrocolloids and sweeteners at 70°C with specific ratios. Agar was dissolved in distilled water at 90°C and added to the concerned mixture. Finally, after moderating pH to 3.4 by adding 40 M citric acid and controlling the Brix degree in constant Brix of 45, the mixture was prepared. The mixture was then poured into stainless steel mesh molds with cavity dimensions of 1.2 cm × 2 cm × 2 cm and the molds were kept at 4°C for 2 hours to form the gel. Then the obtained gel was taken out of the mold cavities and the samples were dried at 70°Cfor 6 hours in a hot air drier with 1.5 m/s airflow rate. Then the regarded tests were performed on the dehydrated samples. To measure pH, pH meter (Hana, Portugal) was used. The measurement of mixture Brix was performed by an optical refract meter (Carlze, Germany). The drying process of the samples was conducted in a hot air drier (Soroush Medicine Co. Iran).Moisture, protein, fiber, lipid, ash, vitamin C, calcium and iron elements were measured according to the Iran national standard.Texture profile analyzer (QTS25 CNS Farnell England) equipped with a software was used to determine the textural properties of the samples. Samples were compressed and decompressed in two reciprocating cycles by a round plate cylindrical probe with 3.5 cm diameter, 1 mm/s probe speed and 5 g force to 30% initial height.Histological properties obtained from force-deformation curve are as follows: Hardness, Cohesiveness, Elasticity, Adhesiveness and Chewiness.Sensory test was performed with the judgment of 10 trained panelists. In order to evaluate the samples. A 9-point Hedonic method (1: very undesirable - 9: very desirable) was used. 5 sensory attributes (color, texture, flavor, odor and overall acceptance) were evaluated.SPSS software was used for the statistical analysis of the parameters. Mean of the replicates were compared via the multi-range Duncan`s test at 95% confidence level.In order to pHotograpHy with Scanning electron microscope, initial preparation should perform on all of samples.The obtained sampleswas removed from the templates and were sectioned on a scale of 2×1×1mm. samples were dehydrated in ethanol solution and then dried in a critical point. Then samples with 30 nm of gold/palladium coated and were tested using SEM (model LEQ1450VP) in the maximum voltage of 15 kv. At least four images with magnification of 500, 1000, 2000 and 5000 were prepared in several different areas of the samples.Results and discussion: The obtained results indicated that the effect of Spirulinawas significant on the moisture content and increasing Spirulinaconcentration led to increased moisture content of the product.The results of texture profile analysis indicated that the hardness and chewness was increased, but the cohesivness of samples was decreased when the amount of Spirulina is increased. The amount of protein, vitamin C, total ash, iron and calcium was increased by increasing the amount of Spirulina. SEM microscopic results showed that adding up Spirulina is led to the reduction of the uniformity of sample’s structure and then the gel matrix will be created with a larger pore. Results of sensory analysis indicate that samples containing 0.25% Spirulina have sensory characteristics (color, aroma, flavor and overall acceptability) than other samples.
Behnam Fiyuzi; Mostafa Mazaheri Tehrani; Esmail Khazaei
Abstract
Introduction: Due to the lack of proper harvest, packaging, transport and storage, about 30% of country dates production cannot be directly absorbed into the consumer market and must be exchanged to valuable products in transformation industries.Hydrocolloids are used in fruit snack formulations to create ...
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Introduction: Due to the lack of proper harvest, packaging, transport and storage, about 30% of country dates production cannot be directly absorbed into the consumer market and must be exchanged to valuable products in transformation industries.Hydrocolloids are used in fruit snack formulations to create novel texture, increase stability due to their water-holding capacity, improve texture and have an impact on flavor release and other structural and sensory properties in the respective products. Gelatin is a gel forming hydrocolloid. Xanthangum is a kind of long-chained polysaccharide with high molecular weight. LikeXanthan and guar gums can also interact with some polysaccharides such as gelatin, agar and carrageenan synergistically leading to increased viscosity or gel forming power. This type of synergistic behavior among polysaccharides is commercially valuable, because it creates a novel texture and a more desirable structure.This study on viscosity and textural changes caused by using a mixture of gums in food formulations is important and will affect the cost of various stages of the process.Material and methods: The initial materials containing date(Shahani variety), condensed whey(brix=35) were provided from Asali Mod compony, Xanthan gum from Sigma company. powdered glucose and date were bought from Mashhad bazar.In order to produce gel based on date puree, the date were fist washed up, peeled and cut into pieces. Then the pieces were grinded. The prepared puree was mixed with hydrocolloids and sweeteners at 70°C with specific ratios. The mixture was then poured into stainless steel mesh molds with cavity dimensions of 1.2 cm × 2 cm × 2 cm and the molds were kept at 4°C for 2 hours to form the gel. Then the obtained gel was taken out of the mold cavities and the samples were dried at Environmental drier with 1.5 m/s airflow rate. In this research, the produce of jelly viable products based on date puree by the different rates of gelatin hydrocolloids in two levels (6 and 8%) and Xanthan in two levels (0.25 and 0.75%) and condensed whey in three levels (5, 10 and 20%) was studied. Dependent variables were consisting of moisture content, water activity, PH, Brix, protein, ash, texture assessment and color parameters. In final has done the sensory evaluation.To measure pH, pH meter (Hana, Portugal) was used. The measurement of mixture Brix was performed by an optical refract meter (Carlze, Germany).Moisture, protein and ash were measured according to the Iran national standard.In order to determine the water activity of the samples, equal weights of the samples were grinded and the water activity was measured by a aw meter (Testo model 200, England) at 20°C. Texture profile analyzer (QTS25 CNS Farnell England) equipped with a software was used to determine the textural properties of the samples. Samples were compressed and decompressed in two reciprocating cycles by a round plate cylindrical probe with 3.5 cm diameter, 1 mm/s probe speed and 5 g force to 30% initial height. Histological properties obtained from force-deformation curve are as follows: Hardness, Cohesiveness, Elasticity, Adhesiveness and Chewiness.In order to measure color parameters of samples, three samples were choose randomized from each formulation, and pictures were taken with 90 angle and pictures were saved with IPG format. The other stages of picture processing were done by ImageJ 1.40g software.Sensory test was performed with the judgment of 10 trained panelists. In order to evaluate the samples. A 9-point Hedonic method (1: very undesirable - 9: very desirable) was used. 5 sensory attributes (color, texture, flavor, odor and overall acceptance) were evaluated.This study was triplicated through a completely randomized design. Gelatin hydrocolloids in two levels (6 and 8%) and Xanthan in two levels (0.25 and 0.75%) and condensed whey in three levels (5, 10 and 20%)were considered as the independent variables and a design composed of 12 formulations was created. SPSS software was used for the statistical analysis of the parameters. Mean of the replicates were compared via the multi-range Duncan`s test at 95% confidence level.Results and discussion: The obtained results showed that with increasing of condensed whey decrease the PH formulation but ash, moisture, water activity and the rate of protein in samples significantly have increased. Also effect of Xanthan and gelatin had an increase trend in water activity of samples. Also with increasing every three variables in formulation, brix had an increase trend. According to results with increasing hardness gelatin has increased the chewing and continuity feature of texture but adhesion had a decreasing trend. The increasing of Xanthan formulation led to increase of elasticity, context chewing of gum and decreasing of samples. Colorimetric results in method of Image processing were not significant on none of colorimetric variables. But in generally with increasing hydrocolloids in formulation had decreasing the light intensity and L*had a decreasing process. Sensory evaluate shows samples containing of 20% whey have less general acceptance. Also the samples containing of highest percentage of hydrocolloids allocated to themselves the less of rating flavors and aromas.
Esmail Khazaei; Fakhri Shahidi; Seyed Ali Mortazavi; Mohebbat Mohebbi
Abstract
In this research, the production of a new kiwi- based product by applying kiwi puree, agar (0.25, 0.5 and 1%), guar (0.25, 0.5 and 1%) and other formulation ingredients (kiwi puree, sweeteners and pH modulators) has been investigated. Dependent variables include moisture content measurement, instrumental ...
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In this research, the production of a new kiwi- based product by applying kiwi puree, agar (0.25, 0.5 and 1%), guar (0.25, 0.5 and 1%) and other formulation ingredients (kiwi puree, sweeteners and pH modulators) has been investigated. Dependent variables include moisture content measurement, instrumental texture analysis and sensory evaluations were carried out in a factorial design and the means were compared at significance level of 5 % by Duncan test. The results indicated that guar had a significant effect on the moisture content of final product. Textural properties of the kiwi pastille were influenced by the type and amount of hydrocolloids. Synergistic effects of agar and guar on the textural properties were also observed. While all products were acceptable regarding sensory properties, the samples prepared with 1% agar and 1% guar obtained the highest score.
